T3+PCR+Procedure

__PCR Procedure__  ﻿__What is PCR?__    ﻿ ﻿ PCR or Polymerase Chain Reaction is used in science for the extraction, amplification and cloning of DNA. The reason for this amplification is so that there will be enough DNA in the extraction to identify the genetic finger print of the DNA. PCR is used to determine likelihood of hereditary diseases, in order to determine the ribosomal characteristics DNA polymerase, "unzips" the double helix that is our genetic code in order to replicate it and produces two sets of the DNA, once the DNA is multiplied many times there is then enough to sequence or use for identification.  We are using PCR to multiply the amount of DNA we have from each lichen sample from the ITS region of the lichen DNA. The ITS region is approximately 561 ribosomal pairs long when cut by the primers we were provided. ﻿ __Uses__    ﻿PCR can be used for a wide range of purposes, in our case it is to amplify the amount of lichen DNA we have in order for it to be sequenced and sent off to genbank  