T2+Resources

=__T2 Resources, Methods, and Materials__ =

Integrated DNA Technologies provided us with the primers ITS1 and ITS4.

New England BioLabs provided us with the 100 base pair DNA ladder and Taq 2X Master Mix.

Faster Better Media LLC provided us with the LB 20X buffer.

Macherey-Nagel provided us with the PCR and DNA extraction clean-up kits.

NCBI (The National Center for Biotechnology), which "advances science and health by providing access to biomedical and genomic information," allowed us to BLAST (Basic Local Alignment Search Tool) sequences in order to find regions of local similarity between sequences. As stated on the website, BLAST "compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families." After we have finished our research, we will deposit our data into the GenBank, which is a genetic sequence database that serves as an annotated collection of all publicly available DNA sequences. GenBank will allow access to our results for years to come.

iBOL.org is the International barcode of life website.

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