Electrophoresis

Electrophoresis is used to separate different size bands of DNA. After we extract the DNA and use PCR to amplify it, we utilize electrophoresis to confirm successfully amplified samples of DNA. Electrophoresis uses the size and charge of DNA molecules to separate them. By running a charge through an agarose gel, labs can separate larger bands of DNA from smaller smaller bands. As a current runs through the gel, the negatively charged DNA moves toward the positively charged end of the gel. The smaller pieces move through the gel more quickly and cover the greatest distance during the time the current is run. In order to view the bands of DNA a dye is added to the amplified sample of DNA. This dye glows brightly under a black lights. To view the results of electrophoresis scientists place the gel under the black light and can take pictures of the gel. Electrophoresis is a common microbiology tool used in many labs to analyze DNA.
 * __Electrophoresis__ ** 

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