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**OVERVIEW OF PROCESS(ES): **

__ Lichen collection: __



__** DNA Extraction: **__

DNA Amplification Through PCR // Through PCR we will obtain many copies of the targeted ITS region. //



__Sequencing:__





//Sequence example://   //**Sample 1-ATTA CGT **// <span style="font-family: arial,helvetica,sans-serif; font-size: 13px; line-height: 19px;">//**<span style="font-family: Georgia,serif; font-size: 15px; font-style: normal; font-weight: normal; line-height: 19px; margin: 0px; padding: 0px;">Sample 2-ATT<span style="background-attachment: scroll; background-clip: initial; background-color: yellow; background-image: none; background-origin: initial; background-position: 0% 0%; background-repeat: repeat repeat;">C CGT **//  <span style="display: block; font-family: Georgia,serif; font-size: 120%; text-align: center;"> <span style="display: block; font-family: Georgia,serif; font-size: 120%; text-align: center;"> This one base pair difference (in this case the base adenine is replaced with a cytosine) identifies the different species of Lichen based on the ITS region used to differentiate species of plants. The ITS region is used to seperate species of Lichen due to its high degree of variability between the sequence of different species. Thus, a difference in the genetic sequence of two samples of Lichen will confirm these two samples would belong to different species. Click here for more background information on DNA barcoding. <span style="display: block; font-family: Georgia,serif; font-size: 120%; text-align: center;"> <span style="display: block; font-family: Georgia,serif; font-size: 120%; text-align: center;">

**<span style="font-family: Georgia,serif;">[|**Deposit in Gen Bank!**] **



<span style="font-family: Georgia,serif; font-size: 120%;">NCBI is the organization that maintains GenBank. By depositing the sequenced ITS region into Genbank, <span style="font-family: Georgia,serif; font-size: 16px; line-height: 23px;">we would offer researchers accessibility by enabling them to obtain the specific ITS region of each sample of fungi we obtained from UConn.

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